Figure 2.

Effect of food intake on protein expression of SPX at tissue level in mice. The feeding experiment was conducted as described in the preceding section with one group as the “fed” group (with food provision at 10:00 am) and the other as the “unfed” group (deprived of food supply). After 6 h with the respective feeding protocols, the liver, omental fat, forestomach and glandular stomach were excised and subjected to IHS staining with a SPX anti-serum (1:600) raised in rabbit (A). Counter staining with hematoxylin was routinely performed and parallel staining with normal rabbit serum (NRS, 1:600) was used as the negative control. Notable changes in SPX staining were not apparent in the liver, omental fat and forestomach but intensification of SPX signals was noted in gastric mucosa of glandular stomach with food intake, especially in the foveolar cells, parietal cells and chief cells of the gastric glands (B). In the same study, tissue lysate was also prepared from the samples freshly collected in the respective tissues and used for measurement of tissue SPX content (C). In the graph presented, the group denoted by an asterisk (*) represents a significant different at p < 0.05 compared to the respective control.