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. 2021 Jul 2;12:681647. doi: 10.3389/fendo.2021.681647

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Copyright © 2021 Wong, Chen, He, Lin, Bian and Wong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Peripheral administration of SPX on food intake in mice. Time course (A) and dose dependence (B) of SPX treatment by IP injection (5 µl/g BW, injected at 10:00 am) on food consumption in mice. For time course study, SPX was tested at a dose of 10 nmol/mouse to examine its effects on food intake up to 8 h. For dose dependence, the corresponding effects of increasing levels of SPX (0.1-10 nmol/mouse) were monitored for 1, 2, and 3 h, respectively. IP injection with physiological saline was used as the control treatment and parallel injection with leptin (10 nmol/mouse) was conducted to serve as a positive control. The groups denoted by asterisks (*)/different letters represent a significant difference at p < 0.05.