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. 2021 Jul 2;12:681647. doi: 10.3389/fendo.2021.681647

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Copyright © 2021 Wong, Chen, He, Lin, Bian and Wong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Receptor specificity for SPX regulation of hypothalamic expression of orexigenic factors and their receptors. IP injection of SPX (5 nmol/mouse) was performed in mice with/without the co-treatment of the GalR2 antagonist M871 (50 nmol/mouse) or GalR3 antagonist SNAP (50 nmol/mouse). The hypothalamus was harvested one hour later after IP injection. Total RNA was isolated and subjected to real-time PCR for transcript expression of NPY (A), AgRP (B), NPY5R (C) and GHSR (D), respectively with β actin mRNA as internal control. The groups denoted by different letters represent a significant difference at p < 0.05.