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. 2021 Jul 2;12:681647. doi: 10.3389/fendo.2021.681647

Figure 7.

Figure 7

Central administration of SPX on hypothalamic expression of orexigenic factors and their receptors. ICV injection of SPX (1 nmole/mouse) was conducted in mice with parallel injection of artificial CSF as control treatment. The hypothalamus was harvested at the time points as indicated and used for total RNA isolation followed by real-time PCR for transcript expression of NPY (A), AgRP (B), MCH (C), ghrelin (D), NPY5R (E) and GHSR (F), respectively. In this study, β actin mRNA was used as the internal control. The groups denoted by different letters represent a significant difference at p < 0.05.