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. 2021 Jul 2;12:681647. doi: 10.3389/fendo.2021.681647

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Copyright © 2021 Wong, Chen, He, Lin, Bian and Wong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Central administration of SPX on hypothalamic expression of anorexigenic factors and their receptors. ICV injection of SPX (1 nmole/mouse) was conducted in mice with parallel injection of artificial CSF as control treatment. The hypothalamus was harvested at different time points and used for total RNA isolation followed by real-time PCR for transcript expression of CART (A), POMC (B), CRH (C), SPX (D), LepR (E) and MC4R (F), respectively. In this study, β actin mRNA was used as the internal control. The groups denoted by different letters represent a significant difference at p < 0.05.