Table 2.

Primer sequences and PCR conditions for RT-PCR of mouse GALR2/3.

Gene target/GenBank accession no	PCR condition				Product size
Sequences of forward (F) and reverse primers (R)	Denaturing	Annealing	Extension	Cycle no.
GALR2/NM_010254.4
F: 5′ CCCTCGATCCAGCCTGTTAAA 3′	94°C	64°C	72°C	×45	113bp
R: 5′ CGGTCTTTAGCTGCAACCCT 3′	45 s	30 s	60 s
GALR3/AF042783
F: 5′ ACCAAGGAGCATCTAGGGCA 3′	94°C	64°C	72°C	×40	111bp
R: 5′ CCCTGTGTTTAGTTGGGTGGA 3′	45 s	30 s	60 s
GAPDH/NM_008084.3
F: 5′ AACTTTGGCATTGTGGAAGG 3′	94°C	64°C	72°C	×40	223bp
R: 5′ ACACATTGGGGGTAGGAACA 3′`	45 s	30 s	60 s