Table 1.

Primers used for genotyping and quantitative RT-PCR

Primer	Sequence	Product size (bp)
Primers for genotyping
Tfr1 loxP	F: 5′-CAG TAA CAG AGG AAT CAT TAG-3′	WT 500 and floxed 650
	R: 5′-CTA AAC CGG GTG TAT GAC AAT G-3′
HSA-Cre	F: 5′-GCC TGC ATT ACC GGT CGA TGC AAC GA-3′	750
	R: 5′-GTG GCA GAT GGC GCG GCA ACA CCA TT-3′
HSA-rtTA	F: 5′-ACT GAG AGG TGG GAA GCT CA-3′	284
	R: 5′-GGC GAG TTT ACG GGT TGT TA-3′
HSA-rtTA,tetO-Cre	F: 5′-AGG TGT AGA GAA GGC ACT TA-3′	412
	R: 5′-CTA ATC GCC ATC TTC CAG CA -3′
Primers for RT-PCR
Transferrin	F: 5′-TGG GGG TTG GGT GTA CGA T-3′	103
	R: 5′-AGC GTA GTA GTA GGT CTG TGG-3′
Tfr1	F: 5′-GTT TCT GCC AGC CCC TTA TTA T-3′	152
	R: 5′-GCA AGG AAA GGA TAT GCA GCA-3′
Tfr2	F: 5′-TTG GGG TCT ACT TCG GAG AGT-3′	179
	R: 5′-GAC AGG AGC CTA AGT GCT CAG-3′
Myod1	F: 5′-CCA CTC CGG GAC ATA GAC TTG-3′	109
	R: 5′-AAA AGC GCA GGT CTG GTG AG-3′
Myogenin	F: 5′-GAG ACA TCC CCC TAT TTC TAC CA-3′	106
	R: 5′-GCT CAG TCC GCT CAT AGC C-3′
Mef2c	F: 5′-ATC CCG ATG CAG ACG ATT CAG-3′	110
	R: 5′-AAC AGC ACA CAA TCT TTG CCT-3′
MCK	F: 5′-CTG ACC CCT GAC CTC TAC AAT-3′	193
	R: 5′-CAT GGC GGT CCT GGA TGAT-3′
Foxo1	F: 5′-CCC AGG CCG GAG TTT AAC C-3′	132
	R: 5′-GTT GCT CAT AAA GTC GGT GC-3′
Actb	F: 5′-GGC TGT ATT CCC CTC CAT CG-3′	154
	R: 5′-CCA GTT GGT AAC AAT GCC ATG T-3′

Actb: Beta-actin; F: forward; Foxo1: forkhead box protein O1; HSA: skeletal muscle actin; MCK: muscle creatine kinase; Mef2C: myocyte enhancer factor 2C; R: reverse; RT-PCR: reverse transcription-polymerase chain reaction; Tfr: transferrin receptor; WT: wild type.