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. 2020 Dec 12;16(7):1317–1322. doi: 10.4103/1673-5374.301034

Figure 2.

Figure 2

Lack of ephrin-A2/A3 enhances progenitor cell potency of Müller cells in vitro.

(A) Relative expression of progenitor cell markers in Müller cells derived from wild-type (WT) and A2–/–A3–/– mice. Note significantly increased levels of Pax6, Chx10, Ngn2, and Sox2 mRNAs in Müller cells of A2–/–A3–/– mice. (B) Quantitation of 5-ethynyl-2′-deoxyuridine (EdU) incorporated Müller cell cultures derived from WT and A2–/–A3–/– mice. n = 6 animals. Statistical analysis was performed by unpaired t-test. Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, vs. the corresponding genes in wild type mice. (C, D) Representative epifluorescence photomicrographs showing the expression of EdU (pink; arrows) in the cultures of Müller cell derived from wild-type (C) and A2–/–A3–/– (D) mice. The nuclei of cultured cells were counterstained by 4,6-diamidino-2-phenylindole dihydrochloride(blue). Scale bar: 20 µm.