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. 2020 Dec 12;16(7):1317–1322. doi: 10.4103/1673-5374.301034

Figure 4.

Figure 4

Retinal cell proliferation in Rho–/– A2–/– A3–/– mice.

(A) Expression of ephrin-A2/A3 and EphA4 mRNA in the retina of 6 weeks old wild-type (WT) mice and Rho–/– mice. *P < 0.05, vs. wild type mice. (B) Quantification of EdU+ cells in retinas derived from 6-week-old WT and Rho–/– mice. Representative photomicrographs showing EdU+ cells (red; white arrows) on retinal sections counter stained by DAPI (blue) and quantification of EdU+ cells in ciliary body (CB), retina and retinal pigment epithelium (RPE) in 6-week-old (C–E) and 12-week-old (F–H) Rho–/– and Rho–/–A2–/–A3–/– mice. Scale bars in C: 20 µm. ***P < 0.001, vs. wild type mice in panel B; *P < 0.05, **P < 0.01, vs. Rho–/– mice in panel E and panel H. (I) Quantification of EdU+ cells in INL, GCL and ONL of 6- and 12-week-old Rho–/–A2–/–A3–/– mice. *P < 0.05, **P < 0.01, vs. 6-week-old Rho–/–A2–/–A3–/– mice. n = 6 animals. Error bar = mean ± SD. Statistical analysis was performed by unpaired t-test. DAPI: 4,6-Diamidino-2-phenylindole dihydrochloride; EdU: 5-ethynyl-2′-deoxyuridine; GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer.