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. 2021 Aug;27(8):920–932. doi: 10.1261/rna.078827.121

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© 2021 Ahmed et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 2. — Loss of EWSR1 inhibits anchorage-independent growth in Ewing sarcoma cells. (A, left) Western assays reveal knockdown for EWSR1 or EWS-FLI1 in A673 cells for treatment with siSCR, siEF, or siEWSR1. (Right) Averaged levels of protein were determined by densitometry relative to siSCR treatments (n = 3). (B, left) Soft agar assays were performed with A673 cells treated with siSCR, siEF, or siEWSR1. (Right) Colonies were counted to reveal reductions in averaged values relative to siSCR treatment (n = 3). (C, left) Western assays reveal knockdown for EWSR1 or EWS-FLI1 in HEK293T/17 cells for treatment with siSCR, siEF, or siEWSR1. (Right) Averaged levels of protein were determined by densitometry relative to siSCR treatments (n = 4). (D, left) Soft agar assays were performed for HEK293T/17 cells treated with siSCR, siEF, or siEWSR1. (Right) Colonies were counted to reveal no change in averaged values relative to siSCR treatment (n = 4). All error bars represent standard deviation. Student's t-test was calculated assuming equal variances: (**) P < 0.01; (*) P < 0.05; n.s., not significant (P > 0.05).