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. 2021 Aug;27(8):920–932. doi: 10.1261/rna.078827.121

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© 2021 Ahmed et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 3. — EWS-FLI1 can alter effects on cell growth for an EWSR1 knockdown. (A, left) Growth on soft agar was assessed for HEK293T/17 cells after transfection of empty plasmids or a plasmid expressing V5-EWS-FLI1. (Right) Colonies were counted to reveal no change in averaged values relative to siSCR treatment (n = 4). (B, left) Soft agar assays were performed with HEK293T/17 cells expressing V5-EWS-FLI1 and cotransfected with siSCR or siEWSR1 (n = 6). (Right) Colonies were counted to reveal a reduction in averaged values relative to siSCR treatment. Error bars represent standard deviation. Student's t-test was calculated for assuming equal variances: (***) P < 0.001.