Fig. 2.

Tie2-targeted Nox2 deficiency exacerbates systemic inflammation and hypotension induced by LPS. Combined EC/L-Nox2KO, Nox2-replete (Nox2Flox), Tie2-targeted Nox2 overexpression (Nox2Tg), and littermate control mice were injected with LPS (10 mg/kg i.v.). (A, E) Median ± interquartile range of clinical scores; (B, F) mean ± SEM of neutrophil sequestration in lungs; (C, G) plasma tumor necrosis factor (TNF)-α levels, 12 h after LPS treatment. (D, H) Systolic blood pressure (BP) at baseline and 6 h after LPS injection. ∗P < 0.05 for indicated comparisons (n = 3–7 per group). 2-way ANOVA followed by Bonferroni's post-test (A, D, E, H), or Kruskal–Wallis followed by Dunn's multiple comparison test (B, C, F, G).