Fig. 4.

Nox2 deficiency in leukocytes and endothelial cells (EC) increases LPS-induced toll-like receptor (TLR4) expression in aorta. Levels of TLR4 mRNA in aorta 12 h after LPS injection (10 mg/kg i.v.) in global Nox2 deficient (Global Nox2KO, A), mice with Tie2-targeted Nox2 deficiency (EC/L-Nox2KO, B), mice with Tie2-targeted Nox2 overexpression (Nox2Tg, C) and chimeric mice with Nox2 deficiency solely in bone marrow cells (L-Nox2KO) or in endothelial cells (EC-Nox2KO) (D). WT indicates wild type. Graphs are mean ± SEM (n = 3–6 per group). ∗P < 0.05 for indicated comparisons. Kruskal–Wallis followed by Dunn's multiple comparison test.