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. Author manuscript; available in PMC: 2021 Jul 16.
Published in final edited form as: J Vis Exp. 2020 Jun 20;(160):10.3791/61242. doi: 10.3791/61242

Figure 5: Examples of ideal filling and common errors during polymer compound infusion.

Figure 5:

(A) When filling endpoint was reached, a robust and fine vascular network was observed. (B) Fully inflated formalin perfused lungs are represented by a white dashed line, (C) Underinflated/deflated lungs are shown. This was observed due to a compromised pulmonary airway. The original inflated position is represented by a white dashed line and the deflated position is represented by a black dotted line, (D) Patchy filling: the vasculature of portions of the lobe remains unfilled while other areas were entirely filled, (E) Incomplete filling: the polymer compound failed to penetrate entire sections of lung, (F) Underfilling: the polymer compound failed to fill distal vasculature, (G) Rupture: the arrow is pointing to the polymer compound extruded from vasculature, (H) Venous filling: note the arrow pointing to the arterial segments entirely filled and extending into the venous system. Veins and venules were of significantly larger caliber, (I) Catheter wedge: Here the catheter was shunted into one artery preventing the vasculature of the right lobes from filling completely while the left lobe was overfilled.