FIG 4.

Compounds do not interact with the intact viral particles. (A) Compound 22 at 50μΜ, 10μΜ, and 2μΜ, as well as the positive control epigallocatechin gallate (EGCG) (1 mg/ml), was mixed with DENV2 stock and passed through a filter. Incubation of compound 22-treated samples on BHK cells resulted in no inhibition of viral infection in each tested condition (formation of viral plaques). Inoculation of cells with EGCG-treated sample demonstrated full inhibition of viral infection. (B) Compound 22 T 10 μΜ was mixed with DENV2 viral stock and further diluted 100 times to infect BHK cells. No suppression of viral infection was noticed in the compound-treated sample, as assessed by the plaque assay. Both positive (virus-infected cells) and negative (noninfected, DMSO-treated cells) controls were included in the assays.