FIG 5.
Sequencing analysis of the viruses used in the cross-resistance assay. (A) NS4B membrane topology and alignment of amino acid sequence at the N-terminal site of the protein in compound 22- and trans-14-resistant virus mutants compared to virus control. An aspartic acid-to-histidine (D31H) nonsynonymous mutation is located at the pTMD1 domain and marked in orange. Passage 16 (P16) of each virus strain was used in the assay. (B) Antiviral activity of several derivatives against the viruses used above (wild-type ZIKV MR766 and compound 22- and trans-14-resistant virus mutants) was evaluated by qRT-PCR in the supernatant of A549 cells at 72 h p.i. Fold shifts in EC50 are indicated in parentheses, while major findings on the activity of some analogues against the trans-14-resistant mutant and wild-type virus are highlighted in yellow.