TABLE 3.

Impact of key Ambler class A, B, C, and D enzymes on activity of CTB/VNRX-5236 and comparators in isogenic strains of E. coli^a

β-lactamase	β-lactamase class	Fold increase in MIC compared to vector control for:b
		CTB	CTB/VNRX-5236 (4)	CTB/CLA	AMX	AMX/CLA	Tebipenem	Sulopenem
TEM-10	A	4	2	4	≥128	8	4	2
CTX-M-15	A	64	0.5	1	≥128	4	4	4
GES-5	A	8	0.5	4	≥128	≥128	512	256
SHV-5	A	128	0.5	1	≥128	8	4	8
SHV-12	A	256	1	1	≥128	8	4	4
VEB-9	A	≥1,024	2	1	≥128	1	2	4
KPC-2	A	16	0.25	16	≥128	≥128	2,048	2,048
KPC-3	A	16	0.25	16	≥128	≥128	2,048	2,048
KPC-3 D179Y	A	32	2	2	4	4	8	16
PER-1	A	1,024	2	2	128	1	4	8
CMY-2	C	1,024	2	1,024	128	≥128	4	8
P99/AmpC	C	≥1,024	4	≥1,024	≥128	≥128	8	16
ACT-17	C	1,024	4	≥1,024	128	≥128	4	8
CMY-42	C	≥1,024	4	1,024	128	≥128	16	16
OXA-23	D	2	0.5	2	≥128	≥128	128	64
OXA-48	D	4	1	4	≥128	≥128	256	512
OXA-163	D	32	0.5	32	≥128	≥128	32	8
OXA-181	D	2	0.5	2	≥128	≥128	256	1,024
NDM-1	B	≥1,024	≥1,024	≥1,024	≥128	≥128	2,048	1,024

^aCTB, ceftibuten; CTB/VNRX-5236 (4), ceftibuten with VNRX-5236 fixed at 4 μg/ml; CLA, clavulanic acid; AMX, amoxicillin; AMX/CLA, amoxicillin with clavulanic acid fixed at 4 μg/ml.

^bMIC increases of ≤8-fold from vector control are shaded in gray and are based on modal MIC testing across 4 replicates. Vector control MICs were CTB, 1 μg/ml; CTB/VNRX-5236, 0.25 μg/ml; CTB/CLA, 1 μg/ml; AMX, 8 μg/ml; AMX/CLA, 8 μg/ml; tebipenem, 0.016 μg/ml; sulopenem, 0.06 μg/ml.