FIG 4.

The anti-NOD2 drug GSK717 blocks the spread of ZIKV infection. (A) Representative confocal imaging (magnification, ×20) showing the antiviral effect of GSK717 at 20 μM and 40 μM. A549 cells were infected with ZIKV (MOI = 1) followed by treatment with DMSO or GSK717 at 20 or 40 μM for 48 h before processing for indirect immunofluorescence. ZIKV-infected cells were identified using a mouse monoclonal antibody (4G2) to the envelope protein and Alexa Fluor 488 donkey anti-mouse to detect the primary antibody. Nuclei were stained with DAPI. Images were acquired using a spinning-disk confocal microscope equipped with Volocity 6.2.1 software. (B) Cellular ATP levels in A549 cells after 48 h of GSK717 or DMSO treatment. Values are expressed as the means from three independent experiments. Error bars represent standard errors of the means.