FIG 8.

Antinodosome drugs enhance the IFN response. A549 cells were treated with GSK583, GSK717, or DMSO alone for 16 h, followed by the addition of human recombinant IFN-α (100 U/ml). Total cellular RNA was harvested for qRT-PCR at 4 and 8 h posttreatment. Relative expression levels of transcripts for eight ISGs (Casp1, GBP5, NLRC5, NLRP3, NLRC4, MX2, IFIT1, and OAS1) in drug- and DMSO-treated cells are shown. Values are expressed as the means from three independent experiments. Error bars represent standard errors of the means. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (by Student’s t test).