Figure 3.
Tools based on nucleic acid synthesis. (a) Overview of oligonucleotide probes that can be generated by chemical and enzymatic DNA synthesis approaches. (b) Genomic profiling of 5-hmC by selective tagging with biorthogonal sugars. (c) Incorporation of photolabile precursors for the generation of apurinic/apyrimidinic (AP) sites in situ. ONV, o-nitroveratrole. (d) DNA nicking and gap-filling strategy for the incorporation of modified oligos into chromatin array DNA substrates.