Skip to main content
. Author manuscript; available in PMC: 2022 Jun 20.
Published in final edited form as: Annu Rev Biochem. 2021 Jun 20;90:287–320. doi: 10.1146/annurev-biochem-080120-021109

Figure 4.

Figure 4

Tools based on small molecule probes. (a) Small molecule probes for activity-profiling of chromatin effectors. (b) Orthogonal probes for profiling epigenetic writers such as histone methyltransferases (HMTs) and histone acetyltransferases (HATs) which use SAM and acetyl-CoA cofactors, respectively. (c) Schematic of the “bump-and-hole” strategy, which can be used to broaden the substrate scope of enzymes for cofactors and inhibitors, as well as to enhance the specificity of chemical probes with their target proteins. (d) Use of heterobifunctional probes to chemically induce the proximity of target proteins and complexes. (e) Bifunctional probes that recruit E3 ligases induce the ubiquitin-mediated proteolytic degradation of target proteins.