FIGURE 2.

A representative deuterium buildup curve (segment 254–255 from peptide 252–255). All exchange times are converted to pH 7, 23°C equivalent using Table S2. The deuteration level (D%) at each time point at each condition is indicated with a colored diamond. The eight sets of deuterium buildup curves for this segment were fitted with eight sets of Equation (3) with a common stretch factor, B (eight k's and one B were optimized at the same time). The optimized B for segment 254–255 was 0.91. ΔG is the free energy change for each formulation compared with unstructured polyalanine using Equation (4a). ΔΔG is the relative free energy in each formulation compared with that for pH 7, 150 mM NaCl obtained by Equation (54b)