TABLE 2.

Average folding free energy, ΔG, and average relative free energy, ΔΔG, of NISTmAb in eight different formulations (kcal/mol)

	pH	150 mM NaCl	mAb	Fab	VH–VL	CH1–CL	CH2	CH3
	5.0	−	−4.1	−4.3	−4.8	−3.8	−3.1	−4.8
	5.0	+	−4.6	−4.7	−5.1	−4.4	−3.8	−5.1
	6.0	−	−5.3	−5.5	−5.9	−5.1	−4.6	−5.9
ΔG	6.0	+	−5.5	−5.6	−6.0	−5.2	−4.9	−5.8
	7.0	−	−5.7	−5.8	−6.3	−5.3	−5.5	−6.0
	7.0	+	−5.9	−6.0	−6.5	−5.6	−5.6	−6.1
	8.0	−	−6.5	−6.6	−7.0	−6.2	−6.4	−6.8
	8.0	+	−6.7	−6.8	−7.2	−6.3	−6.5	−6.9
	5.0	−	+1.9	+1.8	+1.7	+1.8	+2.5	+1.3
	5.0	+	+1.4	+1.3	+1.4	+1.3	+1.9	+1.1
	6.0	−	+0.6	+0.6	+0.6	+0.5	+1.1	+0.2
ΔΔG	6.0	+	+0.5	+0.5	+0.5	+0.4	+0.7	+0.3
	7.0	−	+0.2	+0.3	+0.2	+0.3	+0.1	+0.1
	7.0	+	0.0	0.0	0.0	0.0	0.0	0.0
	8.0	−	−0.6	−0.5	−0.5	−0.6	−0.8	−0.6
	8.0	+	−0.8	−0.7	−0.7	−0.7	−0.9	−0.7

Note: Please see Figure 2 for the definitions of ΔG and ΔΔG.

Abbreviations: C_H, heavy chain constant domain; C_L, light chain constant domain; Fab, antigen binding fragment; mAb, monoclonal antibody; V_H, heavy chain variable domain; V_L, light chain variable domain.