FIGURE 2.
Inhibition of MmALDH4A1 activity by prolines and hydroxyprolines. The assays were performed at room temperature with NAD+ at 1 mM and MmALDH4A1 at 5 μg/ml in a buffer containing 100 mM sodium phosphate pH 7.0 and 10 mM EDTA. The data for each inhibitor were analyzed by global fitting to either the competitive inhibition model (L‐proline, THLP, CHLP, D‐proline, and CHDP) or the uncompetitive inhibition model (THDP) using Origin software