Figure 2.

Lineage relationship between yolk sac progenitor subpopulations

(A) Flow cytometric analysis of Kit⁺ CD41⁺ cells from Csf1r^MericreMerRosa^yfp embryos pulsed at E8.5 (middle panel) and E9.5 (bottom panel) and analyzed 24, 48, and 72 h after. hpi, hours post-induction.

(B) Labeling efficiency (% of YFP⁺ cells) of progenitor subsets after E8.5 (upper) or E9.5 pulse (bottom). OHT at E8.5: n = 5 at E9.5; 9 at E10.5; and 12 at E11.5. OHT at E9.5: n = 6 at E10.5 and 5 at E11.5. Bars represent mean $\pm$ SEM. hpi, hours post-induction.

(C) Labeling efficiency of megakaryocytes (Mk) and red blood cells (RBC) in the yolk sac, blood, and fetal liver after an E8.5 pulse. n = 5 at E9.5; 9 at E10.5; 12 at E11.5; 17 at E12.5; 5 at E13.5; and 2 at E14.5. Error bar respresent SEM.

(D) Labeling efficiency of Mk and RBC in the yolk sac, blood and fetal liver after an E9.5 pulse. n = 6 at E10.5; 5 at E11.5; 10 at E12.5; 5 at E13.5; and 4–8 at E14.5. Error bar respresent SEM. Please also see Figure S2.