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. 2021 May 4;3(1):vdab031. doi: 10.1093/noajnl/vdab031

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© The Author(s) 2021. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Analysis pipeline summary of MetaMorph custom journal and gating on single-cell average intensity. (A) Cells from all tumor and epilepsy cases were pooled on an XY scatter plot based on Iba1 and marker of interest (MOI) average intensity (P2RY12, TMEM119, CD14, or CD163). Gates were applied to contour plots to define microglia and TAM populations by P2RY12 and TMEM119 average intensity (B and C) and to define CD14^high or CD163^high and CD14^low or CD163^low populations (D and E).