Figure 3.

Immunofluorescent identification and gating of microglia and TAM populations using microglial-specific marker expression. Representative immunofluorescent triple labeling using microglial-specific markers P2RY12 with Iba1 and CD14 in grade IV, epilepsy, meningioma, and low-grade tumor tissue (A₁–D₁). Cell population identification by cell-specific marker expression is defined in the figure key. Scale bars = 50 µm, 20 µm zoom. Image analysis and cell-by-cell gating using FCS Express for the above representative cases, with P2RY12 (A₂–D₂) and CD14 (A₃–C₃) plotted against Iba1 average intensity. Representative immunofluorescent triple labeling using microglial-specific markers TMEM119 with Iba1 and CD14 in grade IV, epilepsy, meningioma, and low-grade tumor tissue (E₁–H₁). Image analysis and cell-by-cell gating for representative cases, with Iba1 plotted against TMEM119 average intensity (E₂–H₂) and CD14 average intensity (E₃–H₃).