Fig 4. Whole-genome sequencing of DNA template from the tprA^ko- and wt-SS14 strain harvested at Passage #9 post-transformation.

(A) tprA^ko-SS14 reads assembled to the wt-SS14 genome sequence (NC_021508.1/CP004011.1) showing a gap where the tprA locus previously was. (B) tprA^ko-SS14 reads assembled to the wt-SS14 genome sequence where the tprA locus was replaced in silico with the tp0574promoter-kan^R sequence showing reads aligning to the tp0574promoter-kan^R sequence. (C) Reads from the wt-SS14 genome (sequenced here as control) aligned to the SS14 reference genome (NC_021508.1/CP004011.1) showing the integrity of the tprA locus. (D) Reads from the wt-SS14 genome (sequenced here as control) aligned to the tprA^ko-SS14 genome showing a gap where the tp0574promoter-kan^R sequence is located.