Skip to main content
. 2021 Jul 16;17(7):e1009715. doi: 10.1371/journal.ppat.1009715

Fig 5. Comparison of ACE2 variant susceptibilities to SARS-CoV-2 replication with SARS-CoV-2 spike pseudovirus infection.

Fig 5

A) Tissue culture infectious dose 50 assays, where dilutions of SARS-CoV-2 were added to 96-wells containing HEK 293T cells expressing the denoted WT or variant ACE2 proteins. TCID50 values were normalized to values corresponding to infection of WT ACE2 cells assessed in the same experiment. Samples colored in blue are those where no cytotoxicity was observed in any dilution, and these samples were given a nominal count of 1 to denote the limit of detection of the assay, and were then normalized accordingly. The horizontal dashes indicate geometric means across experiments. Error bars denote 95% confidence intervals. N = 3 or more replicates. B) Scatter plot comparing SARS-CoV-2 pseudovirus infection and geometric means of normalized TCID50 values shown in panel A. Blue lines indicate representative threshold values that separate permissive from non-permissive ACE2 variants. C) Scatter plot comparing published SARS-CoV-2 RBD-sfGFP binding values to geometric means of normalized TCID50 values.