Fig 1. Eb1, Msps and Tau share the same combination of axonal loss-of-function phenotypes in Drosophila primary neurons.

A-H) Images of representative examples of embryonic primary neurons pre-cultured up to 6 days (to deplete maternal gene product; see Drosophila Primary Cell Culture Preparation) and either immuno-stained for Eb1 (top) or for tubulin (bottom); neurons were either wild-type controls (ctrl) or carried the mutant alleles msps¹, tau^KO or Eb1⁰⁴⁵²⁴ in homozygosis (from left to right); asterisks indicate cell bodies, black arrow heads the axon tips, white arrow heads point at areas of MT curling, dashed squares in A-D are shown as 3.5-fold magnified close-ups below each image with black arrows pointing at Eb1 comets; the axonal outline in D is indicated by a dotted line; scale bar in A represents 15 μm in all images. I-N) Quantification of different parameters (as indicated above each graph) obtained from pre-cultured embryonic primary neurons with the same genotypes as shown in A-H. Data were normalised to parallel controls (dashed horizontal line) and are shown as median ± 95% confidence interval (I-M) or mean ± SEM (N); data points in each plot, taken from at least two experimental repeats consisting of 3 replicates each; large open circles in graphs indicate median/mean of independent biological repeats. P-values obtained with Kruskall-Wallis ANOVA test for the different genotypes are indicated in each graph. For raw data see S1 Data.