TABLE 1.

Donor information for MSCs used in the study

ID	Source	Supplier	Sex	Donor age	Passage # at use
Donor 1	Bone marrow	Lonza (commercial)	F	26	5‐7
Donor 2	Bone marrow	Wan‐Ju Li lab (UW‐Madison)	F	25	5‐7
Donor 3	Bone marrow	Lonza (commercial)	F	21	5‐7

Note: Donor 1 MSCs were used for all experiments characterizing differences between 2D monolayer and 3D MSC aggregates, as well as for experiments informing the DOE model. Validation of the DOE model was performed using MSCs from donors 2 and 3.

Abbreviations: DOE, Design of Experiments; MSCs, mesenchymal stromal cells.