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. 2021 Jul 6;17(7):e1009550. doi: 10.1371/journal.pgen.1009550

Fig 4. VqmAPhage residues G201, A202, E207, and M211 are required for binding to Pqtip.

Fig 4

(A) Growth curves of Δtdh ΔvqmAVc V. cholerae harboring phage VP882 vqmAPhage::Tn5 and the indicated 3xFLAG-VqmAPhage alleles in medium lacking (Control) or containing 0.2% arabinose (Induced). (B and C) Normalized reporter activity from Δtdh E. coli harboring (B) PvqmR-lux or (C) Pqtip-lux and the indicated arabinose-inducible 3xFLAG-VqmAPhage alleles. Black, no arabinose; white 0.2% arabinose. Data are represented as mean ± SD (error bars) with n = 3 biological replicates. (D) Close up views of the DBD from the crystal structure of DPO-VqmAVc bound to PvqmR (PDB: 6ide, protein in gray with the HTH motif in yellow, and the DNA in orange). The color scheme for VqmAVc residues G199, A200, Q205, and L209 mirrors that used in panel A.