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. 2021 Jul 6;17(7):e1009550. doi: 10.1371/journal.pgen.1009550

Fig 5. The VqmAVc HTH motif and the immediate C-terminal 25 residues, together, constrain binding to PvqmR.

Fig 5

(A-D) Normalized reporter activity from Δtdh E. coli harboring (A and C) PvqmR-lux or (B and D) Pqtip-lux and arabinose-inducible VqmAVc, VqmAPhage, or the indicated VqmAVc allele. Data are represented as mean ± SD (error bars) with n = 3 biological replicates. Black, no arabinose; white, 0.2% arabinose. (E) Growth curves of Δtdh ΔvqmAVc V. cholerae harboring phage VP882 vqmAPhage::Tn5 and VqmAVc, VqmAPhage, or VqmAVc*171–224 in medium lacking (Control) or containing 0.2% arabinose (Induced).