TABLE 1.
Nucleotide sequences of primers used in single-cell RT-PCR experiments
| Target | Accession no. | Forward | Reverse | Size (bp) |
|---|---|---|---|---|
| STAT3 | NM_011486 | TTCAAGCACCTGACCCTTAG | TGTCTAGCCAGACCCAGAAG | 478 |
| TGATCGTGACTGAGGAGCTG | CTTGGTGGTGGACGAGAACT | 263 | ||
| STAT5a | NM_001164062 | ACTACACCTTCTGGCAGTGG | GTTGGGTGGGTACTAGTTGT | 469 |
| GATGGAGGTGCTGAAGAAGC | CGGTCTGGGAACACGTAGAT | 288 | ||
| SHP2 | NM_001109992 | ACCTCTATGGTGGGGAGAAG | TTTCAGCAGCATTGATACGA | 486 |
| TCCATGGTCACTTGTCTGGA | AATGCTCCACCAGGTCTGTC | 252 | ||
| PI3Kr1 | NM_001024955 | AAGAAGCAGGCAGCTGAGTA | ACGAGGGAGGTGTGTTGATA | 464 |
| GGCAGAAGAAGCTGAACGAG | CCGGTGGCAGTCTTGTTAAT | 265 | ||
| GLAST | NM_148938 | GGTAAAATCGTGCAGGTCAC | CCACACCATTGTTCTCTTCC | 673 |
| ACATGTTCCCTCCCAATCTG | CAAGAAGAGGATGCCCAGAG | 362 | ||
| SNAP25 | NM_011428 | AAGGGATGGACCAAATCAAT | CAATGGGGGTGACTACTCTG | 601 |
| AAAAAGCCTGGGGCAATAAT | AGCATCTTTGTTGCACGTTG | 304 | ||
| T1R3 | NM_031872 | TGCCTGAATTTTCCCATTAT | AGGACACTGAGGCAGAAGAG | 889 |
| CTACCCTGGCAGCTCCTGGA | CAGGTGAAGTCATCTGGATGCTT | 343 | ||
| β-actin | NM_007393 | CCTGAAGTACCCCATTGAAC | GTAACAGTCCGCCTAGAAGC | 943 |
| GGTTCCGATGCCCTGAGGCTC | ACTTGCGGTGCACGATGGAGG | 370 |
Note: Upper: outside primers, Lower: inside primers.
Abbreviations: GLAST, glutamate/aspartate transporter; PI3K, phosphoinositide 3-kinase; SHP2, Src homology region 2 domain-containing phosphatase-2; STAT3, signal transducer and activator of transcription 3; T1R3, taste receptor family 1 member 3.