Fig. 3. Disruption of genes involved in cyclic di-GMP regulation by prophage insertion is responsible for the PAO1 RSCV phenotype.

Representative PAO1 RSCVs were complemented by introducing the wild-type copy of the gene disrupted by prophage insertion. Parent strains (empty vector; pUCP18) and complemented strains were grown on Vogel-Bonner minimal medium, and colony morphology was assessed. RSCV-5 was selected as a representative retS-disrupted RSCV, and RSCV-15 was selected as a representative dipA-disrupted RSCV. PAO1-B11 is the ancestor strain that the RSCVs evolved from. Scale bars, 2 mm.