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. Author manuscript; available in PMC: 2021 Jul 16.
Published in final edited form as: J Autoimmun. 2017 Jun 4;82:85–95. doi: 10.1016/j.jaut.2017.05.007

Fig. 3. Cohousing affects IgA, IgG2b and IgG1 secreting B-cells in the PP and gut lumen.

Fig. 3.

(A), IgA secreting B-cells (CD19+IgA+ B-cells) in the Peyer’s patches were investigated in 12-week old female Nod2−/−NOD and Nod2+/+NOD mice using flow cytometry. Left: A set of representative FACS plots. Right: The graphical summary of the IgA-secreting B-cells in the 4 groups (n = 8, pooled from two independent experiments). (B), IgA concentration in the small intestine of Nod2−/−NOD and Nod2+/+NOD mice cohoused (CH) or non-cohoused (NCH) was measured by ELISA. Data for (B) are from 3 individual experiments (n = 7–8). (C) and (D) IgG2b-secreting B-cells and IgG1-secreting B-cells in the Peyer’s patches were investigated in 12-week old female Nod2−/−NOD and Nod2+/+NOD mice using flow cytometry (n = 4). Student’s t-test was used for statistical analysis. *P < 0.05, **P < 0.01. Error bars represent SEM.