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. Author manuscript; available in PMC: 2022 Jun 3.
Published in final edited form as: Mol Cell. 2021 Apr 9;81(11):2361–2373.e9. doi: 10.1016/j.molcel.2021.03.027

Fig. 2. ToxN is a sequence-specific endoribonuclease.

Fig. 2.

(A) Schematic overview of RNA-seq approach for determining ToxN cleavage sites.

(B) Histograms showing the distribution of the minimum cleavage ratios within well-expressed coding regions (n = 1,717) in E. coli when comparing cells overexpressing ToxN to those harboring an empty vector (red) or comparing two independent replicates harboring an empty vector (blue).

(C-D) Cleavage profiles for four well-cleaved E. coli transcripts. Sites of GAAAU motifs are indicated with red triangles. Well-cleaved instances of GAAAU are highlighted in gray, and a poorly cleaved region containing the motif is highlighted in purple.

(E) Percent of well-cleaved regions containing each possible 5-mer.

(F) Sequence logo for well-cleaved regions.

(G) Histograms showing the distribution of the minimum cleavage ratios within well-expressed coding regions in E. coli with (red, n = 1,195) and without (blue, n = 522) the ToxN motif.

Also see Fig. S2.