FIG 7.

Serum corticosterone levels (A) and mRNA expression of stress-related genes Npy (B) and Y2r (C) in white adipose tissue of cold-stress-induced, high-fat diet-fed mice treated with LAB. Serum collection from NDS groups and HFDS groups was performed at 0, 6, and 12 weeks, and the epididymal white adipose tissue was removed at 12 weeks. mRNA levels were measured by real-time PCR using Gapdh as an internal reference gene. Values represent the mean ± SEM; *P < 0.05, **P < 0.01, and ***P < 0.001 for comparisons between NDS or HFDS groups and respective LAB administration groups; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 for comparisons between ND or HFD and NDS or HFDS groups, respectively (Student’s t test). ×, ND (n = 3) or HFD (n = 3); ○, NDS (n = 5) or HFDS (n = 5); ■, BNR17 (10⁸, n = 5); ▴, LRCC5310 (10⁸, n = 5); ▾, LRCC5314 (10⁸, n = 5).