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. 2021 Jul 1;10:e66687. doi: 10.7554/eLife.66687

Figure 10. IFN signaling restricts EV-D68 replication in primary human airway cells.

Figure 10.

(A, B) Levels of IFN-β (A) or IFN-λ1 (B) as determined by Luminex-based assays in medium harvested from primary human bronchial epithelial (HBE) cells pretreated with the JAK1/2 inhibitor ruxolitinib (5 mM, Ruxo) or DMSO control for 1 hr and then infected with EV-D68 strains MD/09/23229 or KY/14/18953 as indicated in the presence of inhibitor for 48 hr at 33°C. Symbols represent individual biological replicates from at least two unique donors. (C) Induction of the interferon-stimulated genes (ISGs) CXCL10 or IFIT1 in control (DMSO)- or Ruxo-treated HBE infected with the EV-D68 strains MD/09/23229 or KY/14/18953 as assessed by RT-qPCR. Symbols represent individual biological replicates from at least two unique donors. (D) Viral titers in control (DMSO) or Ruxo-treated HBE infected with MD/09/23229 or KY/14/18953 for 48 hr at 33°C. Statistical significance was determined using a Student’s t-test, *p<0.05, **p<0.01, ns, not significant.