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. 2021 Jul 1;10:e66687. doi: 10.7554/eLife.66687

Figure 5. Comparison of EV-D68 strain-specific growth characteristics in basolateral supernatants of primary human airway epithelial cells.

Figure 5.

Primary human bronchial epithelial (HBE) cells grown at an air-liquid interface were infected with 106 PFU of the indicated EV-D68 strains: MD/09/23229 (orange), IL/14/18952 (burgundy), MA/18/23089 (pink), or KY/14/18953 (blue) and incubated at 33°C (A, B) or 37°C (C, D) for the indicated hours post-infection (hpi). HBE were infected from either the apical (A, C) or basolateral (B, D) surfaces. Supernatants were sampled at the indicated hpi from the basolateral compartment and titers determined by TCID50 assays. Titers are shown as mean ± standard deviation from three independent replicates. Dotted line denotes limit of assay detection. (E) Summary table denotes titer at 48 hpi as collected from the basolateral compartment, − indicates no detectable replication, + corresponds to 103, ++ 104, +++ 105, and ++++ 106. Significance determined by two-way ANOVA compared to the KY/14/18953, which exhibited the highest replication levels, ns not significant.