Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57BL/6J | The Jackson Laboratory | Cat# 000664; RRID:IMSR_JAX:000664 | Both sexes used |
| Genetic reagent (Mus musculus) | H11Cas9 | The Jackson Laboratory | Cat# 028239; RRID:IMSR_JAX:028239 | Both sexes used |
| Genetic reagent (Mus musculus) | Rac1fl/fl | Chrostek et al., 2006 | Both sexes used | |
| Genetic reagent (Mus musculus) | Arpc3fl/fl;Ai14 (Rosa26) | Kim et al., 2015 | Both sexes used | |
| Biological sample (Mus musculus) | Primary hippocampal neuron cultures | This paper | Freshly isolated from P0-P1 Mus musculus brains | |
| Biological sample (Mus musculus) | Organotypic hippocampal slices | This paper | Freshly isolated from P3-P8 Mus musculus brains | |
| Cell line (Homo sapiens) |
HEK293T | ATCC | Cat# CRL-3216; RRID:CVCL_0063 | |
| Antibody | Anti-HA (clone 3F10) (rat monoclonal) | Roche | Cat# 11867431001; RRID:AB_390919 | ICC (1:500) |
| Antibody | Anti-HA (clone 16B12) (mouse monoclonal) | Biolegend | Cat# 901501; RRID:AB_2565006 | ICC (1:500) |
| Antibody | Anti-V5 (mouse monoclonal) | ThermoFisher | Cat# R960-25; RRID:AB_2556564 | ICC (1:500) |
| Antibody | Anti-Myc (clone 9E10) (mouse monoclonal) | Santa Cruz | Cat# sc-40; RRID:AB_627268 | ICC (1:250) |
| Antibody | Anti-Bassoon (clone SAP7F407) (mouse monoclonal) | Abcam | Cat# ab82958; RRID:AB_1860018 | ICC (1:400) |
| Antibody | Anti-GFP (chicken polyclonal) | Abcam | Cat# ab13970; RRID:AB_300798 | ICC, IHC (1:500) |
| Antibody | Anti-RFP (rabbit polyclonal) | Rockland | Cat# 600-401-379; RRID:AB_2209751 | ICC, IHC (1:500) |
| Antibody | Anti-RFP (clone 5F8) (rat monoclonal) | Chromotek | Cat# 5f8-100; RRID:AB_2336064 |
ICC (1:500) |
| Antibody | Anti-Homer1 (rabbit polyclonal) | Synaptic Systems | Cat# 160 002; RRID:AB_2120990 |
ICC (1:500) |
| Antibody | Anti-Synapsin1 (guinea pig polyclonal) | Synaptic Systems | Cat# 106 104; RRID:AB_2721082 |
ICC, IHC (1:500) |
| Antibody | Anti-Vgat (guinea pig polyclonal) | Synaptic Systems | Cat# 131 004; RRID:AB_887873 |
ICC (1:500) |
| Antibody | Anti-Gephyrin (mouse monoclonal) | Synaptic Systems | Cat# 147 011; RRID:AB_887717 |
ICC (1:300) |
| Antibody | Anti-NeuN (clone 1B7) (mouse monoclonal) | Abcam | Cat# ab104224; RRID:AB_10711040 | ICC (1:1000) |
| Antibody | Anti-Rac1 (clone 102) (mouse monoclonal) | BD Biosciences | Cat# 610650; RRID:AB_397977 |
EM (1:100), ICC (1:250) |
| Antibody | Anti-ArpC2 (rabbit polyclonal) | MilliporeSigma | Cat# 07–227; RRID:AB_310447 |
EM (1:200) |
| Antibody | Streptavidin Alexa Fluor 555 | ThermoFisher | Cat# S-32355; RRID:AB_2571525 | ICC (1:500) |
| Antibody | Nanogold-Streptavidin | Nanoprobes | Cat# 2016 | EM (1:100) |
| Recombinant DNA reagent | pCMV-EGFP-Synapsin1a (rat) | Gitler et al., 2004b | from George Augustine | |
| Recombinant DNA reagent | pAAV-hSyn-hChR2(H134R)-EYFP | Addgene | Cat# 26973; RRID:Addgene_26973 | |
| Recombinant DNA reagent | pCAG-ChrimsonR-tdTomato | Klapoetke et al., 2014; Addgene | Cat# 59169; RRID:Addgene_59169 | |
| Recombinant DNA reagent | pAAV-hSyn-CaMPARI2 | Moeyaert et al., 2018; Addgene | Cat# 101060; RRID:Addgene_101060 | |
| Recombinant DNA reagent | pAAV-hSyn-BioID2-HA | This paper | see Plasmids | |
| Recombinant DNA reagent | pAAV-hSyn-BioID2-Linker-Synapsin1a-HA | This paper | see Plasmids | |
| Recombinant DNA reagent | pAAV-HiUGE-GS-gRNA vectors | This paper | see Figure 2—source data 1 | |
| Recombinant DNA reagent | pAAV-HiUGE-2xHA-V5-Myc donor vectors | This paper | see HiUGE tagging of candidate genes |
|
| Recombinant DNA reagent | pAAV-hSyn-Cre | This paper | see Plasmids | |
| Recombinant DNA reagent | pAAV-hSyn-W56-GFP-Linker-Synapsin1a | This paper | see Plasmids | |
| Recombinant DNA reagent | pAAV-hSyn-Scr-GFP-Linker-Synapsin1a | This paper | see Plasmids | |
| Recombinant DNA reagent | pCAG-ChrimsonR-tdTomato-P2A-HA-PA Rac1 (DN) | This paper | see Plasmids | |
| Recombinant DNA reagent | pCAG-ChrimsonR-tdTomato-P2A-HA-PA Rac1 (CA) | This paper | see Plasmids | |
| Recombinant DNA reagent | pAAV-hSyn-mEGFP-Rac1 | This paper | see Plasmids | |
| Recombinant DNA reagent | pAAV-hSyn-mCherry-PBD2-mCherry | This paper | see Plasmids | |
| Commercial assay or kit | Mouse neuron nucleofector kit | Lonza | Cat# VPG-1001 | |
| Commercial assay or kit | Pierce Protein A agarose resin | ThermoFisher | Cat# 20333 | |
| Commercial assay or kit | Pierce high capacity NeutrAvidin agarose resin | ThermoFisher | Cat# 29202 | |
| Commercial assay or kit | S-Trap micro kit | Protifi | Cat# K02-micro-10 | |
| Commercial assay or kit | IntensEM | GE Healthcare Life Sciences | Cat# RPN491 | |
| Commercial assay or kit | GoldEnhance EM Plus | Nanoprobes | Cat# 2114 | |
| Chemical compound, drug | Biotin | MilliporeSigma | Cat# B4501; CAS: 58-85-5 |
|
| Chemical compound, drug | SrCl2 | MilliporeSigma | Cat# 255521; CAS: 10025-70-4 |
|
| Chemical compound, drug | Tetrodotoxin citrate (TTX) | Abcam | Cat# ab120055; CAS: 18660-81-6 |
|
| Chemical compound, drug | CdCl2 | MilliporeSigma | Cat# 202908; CAS: 10108-64-2 |
|
| Software, algorithm | ImageJ (FIJI) | Schindelin et al., 2012; Schneider et al., 2012 | RRID:SCR_002285 | Version 1.29, Version 1.52a |
| Software, algorithm | Puncta Analyzer plugin for ImageJ by Barry Wark | Ippolito and Eroglu, 2010 | ||
| Software, algorithm | Simple Neurite Tracer plugin for ImageJ | Longair et al., 2011 | RRID:SCR_016566 | |
| Software, algorithm | Proteome Discoverer | Thermo Scientific | RRID:SCR_014477 | Version 2.2 |
| Software, algorithm | Mascot Distiller and Mascot Server | Matrix Science | RRID:SCR_000307 | Version 2.5 |
| Software, algorithm | Cytoscape | Cytoscape Consortium | RRID:SCR_003032 | Version 3.6 |
| Software, algorithm | getPPIs R package | Bradshaw, 2021 | http://github.com/twesleyb/getPPIs | |
| Software, algorithm | DAVID bioinformatics tools | Dennis et al., 2003 | RRID:SCR_001881 | https://david.ncifcrf.gov |
| Software, algorithm | Ensembl genome browser | Zerbino et al., 2018 | RRID:SCR_002344 | http://uswest.ensembl.org |
| Software, algorithm | CRISPOR guide RNA selection tool | Haeussler et al., 2016 | RRID:SCR_015935 | http://crispor.tefor.net |
| Software, algorithm | pClamp | Molecular Devices | RRID:SCR_011323 | Version 10 |
| Software, algorithm | MiniAnalysis | Synaptosoft | RRID:SCR_002184 | |
| Software, algorithm | MATLAB | MathWorks | RRID:SCR_001622 | Version R2017a |
| Software, algorithm | Prism | GraphPad | RRID:SCR_002798 | Version 8.4 |