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. 2021 Jul 6;10:e64872. doi: 10.7554/eLife.64872

Figure 3. Chemical inhibition of TrkB blocked 7,8-dihydroxyflavone (7,8-DHF)-mediated osteogenesis.

Figure 3.

MC3T3-E1 cells were incubated with or without K252a (100 nM) for 1 hr followed by 7,8-DHF (0.5 μM or 1 μM). (A) K252a suppressed 7,8-DHF-induced proliferation of MC3T3-E1 cells after treatment for 48 hr. (B) K252a suppressed 7,8-DHF-elevated alkaline phosphatase (ALP) activity of MC3T3-E1 cells. (C, D) K252a inhibited 7,8-DHF-induced TrkB phosphorylation in MC3T3-E1 cells. Representative images from three independent experiments are shown in (C). (E-G) K252a inhibited 7,8-DHF-induced activation of Wnt/β-catenin signaling pathway in MC3T3-E1 cells. Representative images from three independent experiments are shown. The expression levels of target proteins in the control group (0 μM 7,8-DHF, without K252a) were normalized to 1. Source files of the full raw unedited blots and blots with the relevant bands labeled were provided in Figure 3—source data 1. All results were expressed as mean ± SD (A-G: n = 3, *p < 0.05, **p < 0.01, ns: not significant, two-way analysis of variance [ANOVA]).

Figure 3—source data 1. The original files of the full raw unedited blots and blots with the relevant bands labeled in Figure 3C–G.
The folders named ‘Figure 3C’, ‘Figure 3D’, ‘Figure 3E’, ‘Figure 3F’ and ‘Figure 3G’ contain the original images in Figure 3C, Figure 3D, Figure 3E, Figure 3F and Figure 3G, respectively (the individual file name containing ‘(labeled)’ is blot with the relevant bands labeled by a red outline).