Figure 4.

Histological distribution of SPX expression in the liver, pancreas and white fat. Immunoreactivity of SPX was detected by IHS in tissue sections (10 µm in thickness) prepared from (A) the liver, (B) pancreas, and (C) white fat of the mouse with SPX antiserum (anti-SPX, 1:600). In these cases, specific signals of SPX could be noted in the hepatocytes, endothelial cells in the central vein of hepatic lobules, endocrine cells in the islets of Langerhans, acinar cells of exocrine pancreas, and adipocytes forming the fat mass. Parallel staining with a similar dilution of normal rabbit serum (NRS) was used as the negative control and counterstaining with hematoxylin was routinely performed after signal development of SPX. The numbers shown on the side of individual panels (×40, ×100, ×200, and ×400) represent the magnification for the respective pictures.