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. 2021 Jul 2;12:681646. doi: 10.3389/fendo.2021.681646

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Copyright © 2021 Wong, He, Sze, Huang, Ko, Bian and Wong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histological distribution of SPX expression in the adrenal gland. (A) SPX immunoreactivity detected by anti-SPX in different regions of the mouse adrenal, including the adrenal capsule, outer cortex and inner medulla. (B) SPX expression in different cell layers of the outer cortex and within the inner medulla. In outer cortex, SPX signals were detected in adrenocortical cells of zona glomerulosa, zona fasciculata and zona reticularis, respectively. Faint signals of SPX immunostaining could also be noted in chromaffin cells of the inner medulla. In this study, parallel staining with NRS was used as the negative control and the numbers shown on the side/in the bottom (×40, ×100, ×200, and ×400) represent the magnification for the respective pictures.