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. 2021 Jul 2;12:681646. doi: 10.3389/fendo.2021.681646

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Copyright © 2021 Wong, He, Sze, Huang, Ko, Bian and Wong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histological distribution of SPX expression in the testes. (A) SPX immunoreactivity detected by anti-SPX in different regions of the mouse testes, including tunica albuginea, seminiferous tubules and interstitial areas with Leydig cells. (B) SPX expression in different cell types within seminiferous tubules. Except for Sertoli cells and spermatogonia, SPX signals could be noted in the spermatocytes, spermatid and mature spermatozoa (Subpanel a-d). In this study, parallel staining with NRS was used as the negative control and the numbers on the side (×40, ×100, ×400, and ×600) are the magnification of the respective pictures.