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. 2021 Jul 12;13:5573–5585. doi: 10.2147/CMAR.S307595

Figure 2.

Figure 2

CircHIPK3 silencing inhibited the malignant cell behaviors and enhanced PTX sensitivity of PTX-resistant BC cells in vitro. (A and B) Relative circHIPK3 expression by qRT-PCR in MDA-MB-231/PTX and MCF-7/PTX cells transfected with si-NC, si-circHIPK3#1, si-circHIPK3#2 or si-circHIPK3#3. (C and D) MDA-MB-231/PTX and MCF-7/PTX cells were transfected with si-NC or si-circHIPK3#3 and exposed to various doses of PTX for 24 h, followed by the measurement of cell viability by CCK-8 assay. MDA-MB-231/PTX and MCF-7/PTX cells were transfected with si-NC or si-circHIPK3#3 and treated with or without PTX (0.5 µM), followed by the assessment of cell colony formation by colony formation assay (E–G), cell cycle distribution by flow cytometry (H–J), cell apoptosis by flow cytometry (K–M). *P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.05, ##P < 0.01, or ###P < 0.001 by a Student’s t-test or ANOVA with Tukey’s post hoc test.