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. 2021 Jul 12;13:5573–5585. doi: 10.2147/CMAR.S307595

Figure 3.

Figure 3

CircHIPK3 directly targeted miR-1286. (A) Sequence of miR-1286, the potential miR-1286-binding sequence within circHIPK3 and the mutation of the target sequence. (B and C) Dual-luciferase reporter assays in MDA-MB-231/PTX and MCF-7/PTX cells using circHIPK3 wild-type (circHIPK3 WT) or mutant (circHIPK3 MUT) luciferase reporters. (D and E) RIP experiments in MDA-MB-231/PTX and MCF-7/PTX cells using anti-Ago2 or anti-IgG antibody. Relative miR-1286 expression by qRT-PCR in 38 pairs of BC tissues and adjacent normal breast tissues (F), BC tissues from 13 primary patients and 25 recurrent patients after treatment based on PTX chemotherapy (G), MCF-10A, MDA-MB-231, MCF-7, MDA-MB-231/PTX and MCF-7/PTX cell lines (H). (I) The expression of circHIPK3 by qRT-PCR in cells transfected with circHIPK3 overexpressing plasmid (circHIPK3) or negative control plasmid (Vector). (J and K) qRT-PCR for relative miR-1286 expression in cells transfected with circHIPK3 overexpressing plasmid (circHIPK3), negative control plasmid (Vector), si-NC, or si-circHIPK3#3. **P < 0.01 or ***P < 0.001 by the Mann–Whitney U-test, Student’s t-test or ANOVA with Tukey’s post hoc test.