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. 2021 Jul 12;13:5573–5585. doi: 10.2147/CMAR.S307595

Figure 4.

Figure 4

CircHIPK3 silencing enhanced PTX sensitivity of PTX-resistant BC cells in vitro through targeting miR-1286. Relative miR-1286 level by qRT-PCR in cells transfected with in-miR-NC or in-miR-1286 (A), si-NC, si-circHIPK3#3, si-circHIPK3#3+in-miR-NC or si-circHIPK3#3+in-miR-1286 (B and C). (D and E) MDA-MB-231/PTX and MCF-7/PTX cells were transfected with si-NC, si-circHIPK3#3, si-circHIPK3#3+in-miR-NC or si-circHIPK3#3+in-miR-1286 and then exposed to various doses of PTX, followed by the detection of cell viability by CCK-8 assay. MDA-MB-231/PTX and MCF-7/PTX cells were transfected with si-NC, si-circHIPK3#3, si-circHIPK3#3+in-miR-NC or si-circHIPK3#3+in-miR-1286 before PTX exposure, followed by the measurement of cell colony formation by colony formation assay (F and G), cell cycle distribution by flow cytometry (H and I), cell apoptosis by flow cytometry (J). **P < 0.01 or ***P < 0.001 by a Student’s t-test or ANOVA with Tukey’s post hoc test.