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. 2021 Feb 3;42(8):1280–1287. doi: 10.1038/s41401-020-00596-y

Fig. 5. PTPL1 inhibits TGF-β signaling and EMT by dephosphorylating p38 MAPK.

Fig. 5

a Immunoprecipitation using the lysates of A549 cells showing the interaction between PTPL1 and p38 MAPK. b Control and PTPL1 knockdown A549 cells were treated with TGF-β1 and/or SB203580, and were subject to Transwell assay for cell invasion (each bar represents the mean ± SEM. **P < 0.01, ***P < 0.001, ****P < 0.0001. ns, non-significant). c–e Control and PTPL1 knockdown A549 cells were treated with TGF-β1 and/or SB203580. The morphological changes of the cells were observed by microscope (c), and the changes of EMT-related proteins were analyzed by Western blot (d, e) (each bar represents the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns, non-significant). f Schematic diagram for PTPL1 regulation of TGF-β signaling in EMT of NSCLC cells.