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. 2021 Jul 16;12:4361. doi: 10.1038/s41467-021-24547-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Relationships between normalized sequence abundance and temperature for six selected protein functional clusters highly linked to the environment (hlePFCs) that were only composed of functionally unannotated sequences. The three graphs on the left, in purple, correspond to the three hlePFCs functionally unannotated in both KEGG and eggNOG databases that had the lowest positions along the second axis of the canonical correspondence analysis (CCA2) (cold and nutrient-rich waters). The three graphs in the middle, in green, correspond to the three hlePFCs functionally unannotated in both KEGG and eggNOG databases that had the highest positions along CCA1 (oligotrophic and warm waters). B Relationships between normalized sequence abundance and location of sampling, whether in the Mediterranean Sea or not, for 3 dark hlePFCs (only functionally unannotated sequences and no taxonomic annotations under the phylum level). These 3 hlePFCs had the lowest positions among dark PFCs on the first axis of the canonical correspondence analysis (CCA2) (correlated to Mediterranean samples). Each boxplot summarizes the abundance values of its focal PFC in Mediterranean samples (n = 6) and non-Mediterranean samples (n = 87). Boxplots minima and maxima correspond to −1.5 and 1.5 times the interquartile range, while the limits of boxes correspond to the first and third quartiles. The centerline indicates the median. All points outside of the minima-maxima range are plotted.